Multiplexed Bead Immunoassays: Advantages and Limitations in Pediatrics

When performing an immunoassay, a specific antibody is used to identify and quantify the concentration of target molecules or analytes in complex samples, such as serum or urine. The use of monoclonal antibodies, highly specific, was a great improvement in these assays. Currently, the combination of these specific antibodies with different fluorophores and novel detection technologies has allowed the achievement of higher sensitivity and several improvements in this technique.